The combined entity will leverage Anghami's advanced infrastructure to enhance the streaming experience, with OSN integrating 18,000 hours of premium content.
The collaboration will result in the first MENA platform to provide video and music, backed by a $50 million cash injection.
As part of the agreement, the Riyadh venture capital firm will also have the option to increase its investment in the future.
The Abu Dhabi-based music streaming service was listed on the Nasdaq back in February of this year.
Thousands of audiobook summaries look set to be added to the portfolio of Anghami after a partnership is announced with leading platform Wajeez.
If everything goes according to plan, the acquisition should help Anghami further increase its growing revenue, which reached $35.5 million in 2021.
The partnership with Rotana is expected to attract even more listeners to Anghami, which already has 75 million users from around the world.
Anghami may soon bring 70 million users into Web 3.0 with its new cryptocurrency token.
Prime subscribers in Saudi Arabia and the United Arab Emirates will automatically get a free six-month subscription to Anghami Plus.
The Dubai venue is scheduled to open in early 2022.